Immunohistochemistry:Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Recombinant:Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Virus:Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Membrane:Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Injection:Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Electron Microscopy:Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
Article Title: Tongue orthotopic xenografts to study fusion-negative rhabdomyosarcoma invasion and metastasis in live animals.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IHC-plusTM Polyclonal Rabbit anti-Human NUMA1/NUMA Antibody (aa900-950) LS Bio Cat# LS-B11047; RRID: AB_2889921 Ki-67 (D2H10) Rabbit mAb Cell Signaling Technology Cat# 9027; RRID: AB_2636984 Myogenin (LO26) Mouse mAb Cell Signaling Technology Cat# 59208 Anti-PAX7 antibody [PAX7/1187] Abcam Cat# ab218472 Recombinant Anti-MyoD1 antibody [EPR6653-131] Abcam Cat# ab133627; RRID: AB_2890928 Alexa Fluor 594 anti-Pan-Cytokeratin Antibody Biolegend Cat# 914206; RRID: AB_2924628 LYVE-1 (E5P8S) Rabbit mAb Cell Signaling Technology Cat# 98947 eFluorTM 450-conjugated CD31 (PECAM-1) Monoclonal Antibody (Clone 390) eBioscience Cat# 48-0311-82; RRID: AB_10598807 Mouse LYVE-1 Alexa Fluor 594- conjugated Antibody R&D systems Cat# FAB2125T Bacterial and virus strains pLV[Exp]-Hygro-CMV>EGFP VectorBuilder N/A pLV[Exp]-Hygro-CMV>Luc2 VectorBuilder N/A Chemicals, peptides, and recombinant proteins D-luciferin PerkinElmer 122799 Hygromycin Millipore Sigma H3274 Matrigel (GibcoTM GeltrexTM LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix) Gibco A1413302 Ketamine Injection VET ONE 510189 Xylazine AnaSed LA Injection VET ONE 510004 Sucrose Sigma-Aldrich S0389 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences 15710 HEPES Buffer, 1M PH 7.3 K.D Medical RGE-3150 Tissue-Tek O.C.T. .. Compound SAKURA 4583 Methylacetamide Sigma-Aldrich M26305 Histodenz Sigma-Aldrich D2158 Triton X-100 Sigma-Aldrich T9284 1-Thioglycerol Sigma-Aldrich 88640 Experimental models: Cell lines RD Kind gift from Dr. Javed Khan N/A JR-1 Kind gift from Dr. Javed Khan N/A SMS-CTR Kind gift from Dr. Javed Khan N/A Experimental models: Organisms/strains NU/NU Nude Mice National Cancer Institute at Frederick N/A Software and algorithms Living Imaging 4.8.0 PerkinElmer https://www.perkinelmer.com/lab-products- and-services/resources/in-vivo-imagingsoftware-downloads.html#LivingImage (Continued on next page) Cell Reports Methods 4, 100802, July 15, 2024 e1
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